pgex4t 1 vector (Cytiva Europe)
Cytiva Europe is a verified supplier 
Cytiva Europe manufactures this product 
94
Structured Review
Cytiva Europe
pgex4t 1 vector
Pgex4t 1 Vector, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex4t-1+vector/pGEX+Vectors+PGEX-4T-1/pm41187848-95-11-14
Average 94 stars, based on 181 article reviews
Pgex4t 1 Vector, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex4t-1+vector/pGEX+Vectors+PGEX-4T-1/pm41187848-95-11-14
Average 94 stars, based on 181 article reviews
pgex4t 1 vector - by Bioz Stars,
2026-09
94/100 stars
Images
Related Articles
Amplification:Article Title: The CTCF insulator protein forms an unusual DNA structure Article Snippet: .. The zinc finger domain of CTCF was amplified by PCR from a mouse CTCF cDNA clone using the primers pr1964 (BamHI) and pr1965 (XhoI) (Table ) and was cloned into the BamHI and XhoI restriction sites in the Polymerase Chain Reaction:Article Title: The CTCF insulator protein forms an unusual DNA structure Article Snippet: .. The zinc finger domain of CTCF was amplified by PCR from a mouse CTCF cDNA clone using the primers pr1964 (BamHI) and pr1965 (XhoI) (Table ) and was cloned into the BamHI and XhoI restriction sites in the Article Title: Role of UPF1-LIN28A interaction during early differentiation of pluripotent stem cells. Article Snippet: Nature Communications | (2024) 15:158 10 To construct GST-tagged LIN28A variants, including full-length (1–209), 1–77, 78–163, 164–209, 1–124, and 125–209, the vector, pGEX4T-1 (GE Healthcare Life Sciences), and PCR-amplified product from pFLAG-LIN28A using primers LIN28A_XhoI-F and LIN28A_NotI-R for 1–209, LIN28A_XhoI-F and LIN28A(1–77)_NotI-R for 1–77, LIN28A(78–163)_XhoI-F and LIN28A(78–163)_NotI-R for 78–163, LIN28A(164–209)_XhoI-F and LIN28A_NotI-R for 164–209, LIN28A_XhoIF and LIN28A(1–124)_NotI-R for 1–124, and LIN28A(125–209)_XhoI-F and LIN28A_NotI-R for 125–209 were digested with XhoI (NEB, R0146) and NotI (NEB, R0189) and ligated. .. To construct pGEX-GST-MYC-UPF1 variants, including fulllength (1–1118), 1–244, 295–418, 419–700, and 701–914, the Clone Assay:Article Title: The CTCF insulator protein forms an unusual DNA structure Article Snippet: .. The zinc finger domain of CTCF was amplified by PCR from a mouse CTCF cDNA clone using the primers pr1964 (BamHI) and pr1965 (XhoI) (Table ) and was cloned into the BamHI and XhoI restriction sites in the Article Title: Phosphatidylinositol 5-Phosphate Links Dehydration Stress to the Activity of ARABIDOPSIS TRITHORAX-LIKE Factor ATX1 Article Snippet: .. Phosphoinositide 3-phosphatase assays were performed using the malachite green assay , Clones obtained as described above were introduced into the Article Title: The B56γ3-containing protein phosphatase 2A attenuates p70S6K-mediated negative feedback loop to enhance AKT-facilitated epithelial-mesenchymal transition in colorectal cancer Article Snippet: .. All expression vectors for 4HAB56γ3 were constructed as reported previously [ ]. pGEX4T-1-FLAG-p70S6K1 was prepared by excising cDNA encoding the FLAG-p70S6K from pcDNA3.1-FLAG-p70S6K1, which was subsequently cloned into the Article Title: New insights of rotenone targeting cordycepin synthetase 1-cordycepin synthetase 2-glyceraldehyde-3-phosphate dehydrogenase (Cns1-Cns2-GAPDH) triple complex to enhance cordycepin synthesis in Cordyceps militaris. Article Snippet: Cordycepin isolated from Cordyceps militaris is an adenosine analogue with various biological activities, and its synthesis is dependent on cordycepin synthetase 1 (Cns1) and cordycepin synthetase 2 (Cns2).. Our previous study showed that rotenone induction promotes cordycepin synthesis by regulating energy metabolism pathways such as Embden-Meyerhof-Parnas pathway (EMP).. However, the exact molecular mechanism remains unclear. Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells. Article Snippet: .. Cloning, Expression, and Protein Purification The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells Article Snippet: .. The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Malachite Green Assay:Article Title: Phosphatidylinositol 5-Phosphate Links Dehydration Stress to the Activity of ARABIDOPSIS TRITHORAX-LIKE Factor ATX1 Article Snippet: .. Phosphoinositide 3-phosphatase assays were performed using the malachite green assay , Clones obtained as described above were introduced into the Plasmid Preparation:Article Title: Phosphatidylinositol 5-Phosphate Links Dehydration Stress to the Activity of ARABIDOPSIS TRITHORAX-LIKE Factor ATX1 Article Snippet: .. Phosphoinositide 3-phosphatase assays were performed using the malachite green assay , Clones obtained as described above were introduced into the Article Title: The B56γ3-containing protein phosphatase 2A attenuates p70S6K-mediated negative feedback loop to enhance AKT-facilitated epithelial-mesenchymal transition in colorectal cancer Article Snippet: .. All expression vectors for 4HAB56γ3 were constructed as reported previously [ ]. pGEX4T-1-FLAG-p70S6K1 was prepared by excising cDNA encoding the FLAG-p70S6K from pcDNA3.1-FLAG-p70S6K1, which was subsequently cloned into the Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells. Article Snippet: .. Cloning, Expression, and Protein Purification The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells Article Snippet: .. The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Article Title: Role of UPF1-LIN28A interaction during early differentiation of pluripotent stem cells. Article Snippet: Nature Communications | (2024) 15:158 10 To construct GST-tagged LIN28A variants, including full-length (1–209), 1–77, 78–163, 164–209, 1–124, and 125–209, the vector, pGEX4T-1 (GE Healthcare Life Sciences), and PCR-amplified product from pFLAG-LIN28A using primers LIN28A_XhoI-F and LIN28A_NotI-R for 1–209, LIN28A_XhoI-F and LIN28A(1–77)_NotI-R for 1–77, LIN28A(78–163)_XhoI-F and LIN28A(78–163)_NotI-R for 78–163, LIN28A(164–209)_XhoI-F and LIN28A_NotI-R for 164–209, LIN28A_XhoIF and LIN28A(1–124)_NotI-R for 1–124, and LIN28A(125–209)_XhoI-F and LIN28A_NotI-R for 125–209 were digested with XhoI (NEB, R0146) and NotI (NEB, R0189) and ligated. .. To construct pGEX-GST-MYC-UPF1 variants, including fulllength (1–1118), 1–244, 295–418, 419–700, and 701–914, the Activity Assay:Article Title: Phosphatidylinositol 5-Phosphate Links Dehydration Stress to the Activity of ARABIDOPSIS TRITHORAX-LIKE Factor ATX1 Article Snippet: .. Phosphoinositide 3-phosphatase assays were performed using the malachite green assay , Clones obtained as described above were introduced into the Expressing:Article Title: The B56γ3-containing protein phosphatase 2A attenuates p70S6K-mediated negative feedback loop to enhance AKT-facilitated epithelial-mesenchymal transition in colorectal cancer Article Snippet: .. All expression vectors for 4HAB56γ3 were constructed as reported previously [ ]. pGEX4T-1-FLAG-p70S6K1 was prepared by excising cDNA encoding the FLAG-p70S6K from pcDNA3.1-FLAG-p70S6K1, which was subsequently cloned into the Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells. Article Snippet: .. Cloning, Expression, and Protein Purification The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Construct:Article Title: The B56γ3-containing protein phosphatase 2A attenuates p70S6K-mediated negative feedback loop to enhance AKT-facilitated epithelial-mesenchymal transition in colorectal cancer Article Snippet: .. All expression vectors for 4HAB56γ3 were constructed as reported previously [ ]. pGEX4T-1-FLAG-p70S6K1 was prepared by excising cDNA encoding the FLAG-p70S6K from pcDNA3.1-FLAG-p70S6K1, which was subsequently cloned into the Article Title: Role of UPF1-LIN28A interaction during early differentiation of pluripotent stem cells. Article Snippet: Nature Communications | (2024) 15:158 10 To construct GST-tagged LIN28A variants, including full-length (1–209), 1–77, 78–163, 164–209, 1–124, and 125–209, the vector, pGEX4T-1 (GE Healthcare Life Sciences), and PCR-amplified product from pFLAG-LIN28A using primers LIN28A_XhoI-F and LIN28A_NotI-R for 1–209, LIN28A_XhoI-F and LIN28A(1–77)_NotI-R for 1–77, LIN28A(78–163)_XhoI-F and LIN28A(78–163)_NotI-R for 78–163, LIN28A(164–209)_XhoI-F and LIN28A_NotI-R for 164–209, LIN28A_XhoIF and LIN28A(1–124)_NotI-R for 1–124, and LIN28A(125–209)_XhoI-F and LIN28A_NotI-R for 125–209 were digested with XhoI (NEB, R0146) and NotI (NEB, R0189) and ligated. .. To construct pGEX-GST-MYC-UPF1 variants, including fulllength (1–1118), 1–244, 295–418, 419–700, and 701–914, the Article Title: Rational mutagenesis to support structure-based drug design: MAPKAP kinase 2 as a case study Article Snippet: .. Most human MK2 constructs were engineered as fusion proteins with Schistosoma japonicum glutathione S -transferase (GST; ), using the shRNA:Article Title: The B56γ3-containing protein phosphatase 2A attenuates p70S6K-mediated negative feedback loop to enhance AKT-facilitated epithelial-mesenchymal transition in colorectal cancer Article Snippet: .. All expression vectors for 4HAB56γ3 were constructed as reported previously [ ]. pGEX4T-1-FLAG-p70S6K1 was prepared by excising cDNA encoding the FLAG-p70S6K from pcDNA3.1-FLAG-p70S6K1, which was subsequently cloned into the Cloning:Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells. Article Snippet: .. Cloning, Expression, and Protein Purification The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Protein Purification:Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells. Article Snippet: .. Cloning, Expression, and Protein Purification The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Multiple Displacement Amplification:Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells. Article Snippet: .. Cloning, Expression, and Protein Purification The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells Article Snippet: .. The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Virus:Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells. Article Snippet: .. Cloning, Expression, and Protein Purification The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the Article Title: Inhibitors against Two PDZ Domains of MDA-9 Suppressed Migration of Breast Cancer Cells Article Snippet: .. The PDZ1 domain (residues 113–192), PDZ2 domain (residues 197–273) and tandem PDZ structural domain (residues 113–273) of MDA-9 were cloned into the |